What Is Column Chromatography Types Apllication

Column Chromatography

chromatography image

 

INTRODUCTION

Column chromatography is type of chromatography widely used in pharmaceutical and mainly analysis of samples.

in Column Chromatography the stationary phase  is a column and mobile phase is liquid or gas.

PRINCIPLE INVOLVED IN Column Chromatography  

Various type of principle involved in chromatography as follows

• Adsorption        
• Partiotion          
• Ion exchange     
• Molecular sieving 

principle effect the stationary phase given with example

• Adsorption : stationary phase is silica gel.       
• Partiotion    : stationary phase is liquid coated        
• Ion exchange  : stationary phase is ion exchange resin    
• Molecular sieving : stationary phase is Gel marix

THEORIES OF Column Chromatography  

Two types of theries involved in column chromatography

1. Plate theory
2. Rate theory

 Plate theory
According to this theory the sepration efficiency of column is depend on number of plates attached to it.
Mean greater no. of plates result to higher efficiency of sepration 
express by formula as follows 

H=L/N 

Where the                                    

L is length of column  

H is plate height         

N is no. of plates        

 

Rate theory

This theory is explain the effect of parameter that affect the width of column band.
the path length solvent moves form  top of thecolumn and time to reach the boettom of column also effect the width of bond
this theory well explain by van deemter equation
  
EFFICIENCY OF COLUMN   

AS WE STUDY EARLIER THE rate and plate theory explain about efficiency  of column

so revised that efficiency of column no. of plates increses sepration efficiency

thats whynyou want to seprate rapidly use multiple plate each times.

Column packing technique

 chromatographic seperation ccolumn avilable in various size ranges from few cm tometers 

column prepared by uding silica gas tubes such as burrette 

two type of packing involved as follows 

WET PACKING 

DRY PACKING 

APPLICATION

Chromatography has wide range of application some major given below 

1. for the identification of pharmaceuticals substance and prepratio n 
2. test for homogenity ofsubstance 
3. determination of individual substances
4. it has got excellent application in pesticide analysis
5. applicable also for the analysis of glycoside and carbohydrate
6. anlysis of vitamins done sucessfully